2) In “Chardakov method for water potential measurement” experiment, you find that M solution has not been changed by water loss or absorption by the. Chardakov Technique. Plant Physiology. UNI. Incubating tissue in solution. Pre- incubation. Incubation. Post-incubation. Save for later measurement. Did solution change concentration? Solutes stay the same If water left or entered tissue –Water also entered or left solution –Solution becomes more dilute or.

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If necessary, add more of the appropriate solution to completely submerge the cores but the final volume in mdthod tube must be the same. Boy, Oh Buoyancy Does it Float?

Chardakov method

Does it correspond to the value obtained by the Chardakov technique? Work quickly to minimize evaporation and keep the tissue wrapped in a moist towel. Be sure to use a different pipet for each dye stock.

Record the temperature of the solutions Table 1 Using a Pasteur pipet, remove a small amount of water dyed with methylene blue to dye the sucrose solution, dip a dry probe chardaakov methylene blue powder and then mix.

In contrast to the Chardakov method which analyzes changes in solution density after incubation, this technique monitors tissue weight changes. If you wish to download it, please recommend it to your friends in any social system.

The water potential of the tissue is considered to be equal the osmotic potential of the incubating solution at which there is no change in tissue weight i. Slowly release a drop of the methylene blue solution from the pipette and note whether the drop of the dye sinks, mrthod, or floats to the surface in this solution merhod subjectively estimate whether it does so rapidly or slowly. Repeat for all solutions.


The Chardokov method provides a quick means to determine plant tissue water potentials. Alternately, for a more accurate measurement of changes in the solution density, a refractometer can be used.

Auth with social network: Put two or preferably three potato cores in each solution water or sucrose. Change in weight of potato cores incubated in sucrose solutions. Then, the percent change in weight of the tissue is plotted versus solution concentration or osmotic potential. Work quickly to minimize evaporation and keep the tissue wrapped in a moist towel. From the graph, determine the concentration of the sucrose solution in which there was no net weight gain i.

Periodically swirl the containers. Does a gas differ from other fluids? Mix the tubes thoroughly with a vortex mixer.

The answer will methoe in Jm -3 which are equivalent to pressure Pa. Use a cork borer to prepare at least 27 uniform tissue samples from the potato. Share buttons are a little bit lower. Which method do you think will be more accurate? Divide by 10 6 to convert to MPa. An alternate method to determine his point requires performing a regression analysis of the best fit line of your data.

What would happen to The size of the tissue? Registration Forgot your password? Record your data in Table 2. Weigh the cores to the nearest 0. We think you have liked this presentation.


If the density of a solution does not change no net movement of water then this solution has the same water potential as the tissues nethod were incubated in it. The solution gains or looses water depending on the water potential of the tissue.

Plant Physiology

Water potential values determined by this method may be slightly more negative than those obtained by the Chardokov method. No change in density, concentration No net water movement Tissue and solution were at the same WP If we can find this solution concentration —We can find the tissue WP. To use this website, you must agree to our Privacy Policyincluding cookie policy. This is due to incipient plasmolysis that occurs at low solution water potential.

Complete Tables 1 and 2. Published by Sabrina Grant Modified over 2 years ago. In both techniques, tissue samples are incubated in a series of solutions of known osmotic water potential. It is assumed that solute movement between tissue and solution is negligible. Be sure not to include any fragments of the skin. One distinct advantage of this technique is that it provides a more accurate estimate of water potential. Use a cork borer to prepare at least 27 uniform tissue samples from the potato.

Incubate the cores for at least 1. Determine the approximate sucrose concentration for which there is no net change in density after tissue incubation i.