1. Eur J Clin Microbiol Infect Dis. Jun;35(6) doi: /s Epub Mar Since influenza viruses can cause severe illness, timely diagnosis is important for an adequate intervention. The available rapid detection methods either lack. The Solana Influenza A+B Assay is a rapid qualitative in vitro diagnostic test for the detection and differentiation of influenza A and influenza B viral RNA in nasal .

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The applicability of this multiplex PCR was evaluated with 27 influenza virus A and 9 influenza virus B reference strains and isolates. Influenza A is further divided into different subtypes. Besides being rapid, this method also has the advantage of a standardized protocol that can be applied easily to other respiratory viruses; the TaqMan PCR can be performed under uniform amplification conditions, thereby allowing the use of target-specific primer inflkenza probe sets. However, it can be broken down further into specific viral lineages and strains.

Rapid diagnostic tests with increased sensitivity are essential for the reliable detection of influenza A and B and enable immediate, effective treatment decisions. Influenza C is regarded as the least serious of the three types that humans can contract.

From worsening anxiety to making depression more likely, sugar is seriously harmful to your mental health. Longitudinal follow-up of six patients with either influenza virus A patients 3 to 6 or virus B infection patients 1 and 2.

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All of the influenza virus strains but none of the other respiratory viruses were detected. The sensitivity of the multiplex assay was determined in two ways: Both influenza A and influenza B are extremely contagious.


One milliliter of the supernatant z used directly for virus culturing. For example, influenza A H3N2 viruses are associated with more hospitalizations and deaths in children and the elderly than in other age groups, according to the CDC. In addition, the procedure is less complicated than other RT-PCR methods, and the chances of contamination are minimized because there is no post-PCR processing of the samples.

Primers and probes were selected from highly conserved regions of the matrix protein gene of influenza virus A and the hemagglutinin gene segment of influenza virus B.

Simultaneous Detection of Influenza Viruses A and B Using Real-Time Quantitative PCR

This type of analysis, however, is time-consuming, either because sub type-specific PCRs need to be performed or because the post-PCR analysis is complicated. The flu, or influenza, is a contagious respiratory illness caused by viruses that infect the nose, throat, and sometimes the lungs. Multiplex detection of four pathogenic retroviruses using molecular beacons. Comparison of reverse transcription-PCR with tissue culture and other rapid diagnostic assays for detection of type A influenza virus.

Recently published results of clinical trials using new anti-influenza virus compounds, the neuraminidase inhibitors, demonstrated that these drugs are effective against influenza viruses A and B and are most effective when administered early, when symptoms first emerge 68 You can also contract the virus by touching a surface that has the virus on it and then touching your nose or mouth.

Support Connect with us. Lower Your Risk of Getting the Flu. While most confirmed infections during flu season will be influenza A, the occurrence of influenza B infections can increase late in the flu season.


Even though influenza virus infection usually persists for only 1 week, quantification might be a useful tool for evaluating the effects of antiviral therapy. Over-the-counter medications can be taken to relieve nasal congestion, fever, and aches and pains.

Simultaneous Detection of Influenza Viruses A and B Using Real-Time Quantitative PCR

Type and subtype specific detection of influenza viruses in clinical specimens by rapid culture assay. Some of these specimens were obtained at regional general practices participating in a study to evaluate the efficacy of influenza vaccination. Learn the Side Effects. Real-time quantitative PCR for the detection of minimal residual disease in acute lymphoblastic leukemia using junctional region specific TaqMan probes.

First-round amplification primers and nested primers were selected from conserved regions of the gene for the matrix protein of influenza virus A first-round primer set: Types of flu virus.

For full functionality of this site it is necessary to enable JavaScript. PCR products were visualized on an ethidium bromide-stained agarose gel using UV illumination. Influenza D is found mainly in cattle.

The method can generate results within 4 to 5 h and does not require any post-PCR processing 910 ,