Introducción a la HPLC: aplicación y práctica. Front Cover. Oscar Alberto Quattrocchi. Artes Gráficas Farro, – pages. 6 oct. We are always happy to assist you. Document Description. Introduccion a La HPLC Quattrocchi. Document Share. Documents Related. Results: the HPLC method described for the simultaneous determination of sulphadoxine and pyrimethamine in mL .. Quattrocchi OA, De Andrizzi SA, Laba RF. Introducción a la HPLC Aplicación y. Práctica. Buenos Aires: Artes Gráficas.

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Accordingly, a method for the determination of sulfamethazine was developed using differential pulse voltammetry, at pH 11 and with an accumulation time of 3 min. Liquid-liquid extraction yields a good recovery of nifedipine from serum.

Introducción a la HPLC: aplicación y práctica – Oscar Alberto Quattrocchi – Google Books

The solutions were protected from light with an aluminum foil wrapping and usually freshly prepared solutions were used. Ngila, Talanta 58, Subscribe to our eNewsletter with daily, weekly or monthly updates: Table 3 summarized all of validated values.

Med, Care44 Tue Aug 17, 7: It includes a liquid-liquid extraction, and carbamazepine as internal standard. The analysis is fast, and the method allows a large number of samples to be measured simultaneously.

Introduccion a La HPLC Quattrocchi

The nifedipine concentration ranged between 0. Precision was calculated by RSD and accuracy was measured from percentage recoveries obtained by the analysis of the quality control samples, determined by linear regression equation of peak area vs. Detection and quantification limits Serum spiked with nifedipine standard to obtain concentrations of 0.


All chemicals and inttoduccion were of analytical grade. Services on Demand Journal.

Sun Mar 18, 1: Drug-free serum used for the validation of the method was obtained from healthy volunteers. Detection and quantification limits.

Precision and accuracy Quality control samples spiked at three concentrations 0. Bioanalytical Method Validation, U. The selectivity of the method was examined by preparing and analyzing checked by analyzing human serum samples spiked with nifedipine and quattrochi internal standard carbamazepine. Nifedipine is used alone or in combination with other classes of antihypertensive agents in the management of hypertension.

Iowa State University Press, Best regards, Dmitriy A. The accuracy and precision did not exceed 4.

The developed modified electrode was characterized using cyclic voltammetry and scanning electron microscopy. How to cite this article. The best mobile phase was a mixture of chloroform: The chromatographic conditions were arrived at after investigating several parameters, such as detection wavelength, ideal mobile phase and their proportions, internal standards, and optimal conditions for sample extraction. Thank you guys, I’ll try to find them to buy, here in Brazil we do not have many options, only a couple of books by local authors, but they treat the basics only.


Methods Standard solutions Nifedipine was dissolved with chloroform: Nifedipine was dissolved with chloroform: But, I will read this book too, thanks.

Chloroform, ethyl acetate, cyclohexane and introdufcion were obtained from Merck Darmstadt, Germany. The more common ways to evaluate the adherence of the patients with their medication are questionnaires about the daily administration of the drug, and pill counts, with the problem that the intrroduccion can be true or false.

Sample application was done on 4 mm bands.

Therefore, the principal advantage of HPTLC is a fast analysis, with a high selectivity, accuracy, sensitivity nanograms in UV absorbance mode and picograms in fluorescence mode and precision. Fri Jan 19, Care37 In patients with normal renal and hepatic function, the plasma half-life of nifedipine is about 2 h when administered as conventional capsules, and about 7 h when administered as extended-release tablets.

Pharmaceutics, For determination of nifedipine in blood, some methods using HPLC, voltammetry and spectrophotometry 39,40 have been reported. Three replicates each were prepared and each replicate was applied as bands three times.